The platform
Powered by Nanopixels™
PCR, unleashed.
More targets. No compromises.
Up to
200
plex
One reaction.
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HYPERPLEX PCR™
PCR. Beyond limits.
200 targets. One reaction.
Hyperplex PCR combines high multiplexing, single-base specificity and ultra-sensitive molecular counting in one seamless, single-reaction workflow.
See the bigger picture
Wider multiplexing. Deeper counting.
Move beyond a handful of markers. Measure up to 200 targets with single-base specificity and ultra-sensitive molecular counting.
See what makes hpPCR unique01 Multiplex unleashed / powered by Nanopixels™
200 targets. No sample splitting.
Resolve many biomarkers in the same reaction while preserving independent target quantification.
02 Specificity / Padlock probes
See the difference of one base.
Ligase-driven recognition enables single-nucleotide discrimination, helping distinguish closely related sequences.
03 Quantification / Molecular counting
Count molecules, not intensity.
Each amplified molecule becomes a discrete count, enabling ultra-sensitive and precise digital quantification.
04 Scale / Standard formats
Built to move through the lab.
Standard plate formats. Familiar lab equipment. Fully automated with Hamilton. Bring high-plex analysis into everyday lab operations.
The Aplex platform
Your lab. Your way. One platform.
Hyperplex PCR™
Flexible by design
5 samples or 96. Keep it cost-efficient.
Run the samples you have. No need to wait for a full plate.
Up to
192
samples per day
96-well format
DNA and RNA together. Reverse transcription included.
DeepCount™
In development
Bring rare variants into view.
30M+
Molecular counts per well
<0.001%
VAF sensitivity
Focused analysis for rare variants against abundant background.
6-target configuration · Preliminary specifications, subject to change before launch.
Tracium™
Add-on
A reference for every target.
Self-correcting
Reduce bias and inhibition.
Direct quantification
No calibration curves needed.
Standardized
Reproducible across sites.
Sample utilisation
Put the full aliquot to work.
No dead volumes. The full sample aliquot participates in the reaction.
Automated workflow, 15 min hands-on.
Standardized, scalable workflows for reproducible results across runs and labs.
Aplex scanner
Coming soon
Dedicated readout. Built for Aplex.
Our own scanner for the platform’s molecular readout.
Analysis software
Automated analysis & QC.
Designed for controlled workflows and to support GxP and 21 CFR Part 11 requirements.
Sample Multiplex
Add-on
More samples. From every plate.
Samples in each pool share one hpPCR workflow cost.
Targets per sample: 2 (default selection).
Up to
50
samples per well
96-well plate
4,800
samples per plate
384-well plate
19,200
samples per plate
PLATFORM COMPARISON
Analysis without compromise.
| qPCR Quantitative PCR | dPCR Digital PCR | NGS Targeted sequencing | hpPCR Hyperplex PCR™ | |
|---|---|---|---|---|
| Multiplexing | Typically 1–6 per reaction | Up to 12 per reaction | Targeted panels 10s–1,000s+ per sample | Up to 200 per reaction |
| Quantification | Fluorescence Relative / standard curve | Partitions Poisson-corrected | Sequence reads Molecule counts with UMIs | RCP counts Without partitions |
| Readout capacity | N/A No discrete counts | Typically Tens of thousands partitions / reaction | Sequencing dependent reads / sample | 1M+ counts/well30M+ with DeepCount™ |
| Single-base resolution | Assay-specific Allele-specific probes | Assay-specific Allele-specific probes | Read sequence Within covered regions | 1-base discrimination Padlock ligation |
| Time to results | Same-day Workflow dependent | Same-day Workflow dependent | Multi-day Faster workflows exist | Same-day From extracted DNA / RNA |
| Precision | 50–100% CV Assay dependent | ~10% CV Assay dependent | 10–30% CV Library prep/read depth dependent | <10% CV With Tracium™ |
EVIDENCE IN PRACTICE
Real samples. Published results.
All resourcesCell typing across two forensic labs.
A proof-of-concept 17-plex mRNA assay was evaluated with body-fluid samples and mixtures. Detection of expected markers was comparable to or better than the CE-based CellTyper method.
Forensic Science International: Genetics · 2026
National Forensic Centre, Sweden; Netherlands Forensic Institute; Lund University.
Read publication: Hyperplex PCR for forensic mRNA-based cell typingTwenty targets. One gene-expression assay.
A published protocol uses hpPCR to investigate wheat transcription factors under salt stress. Expression trends agreed with RT-qPCR across the measured targets.
Methods in Molecular Biology · Published online 2025
University of Gothenburg, with Aplex Bio.
Read protocol: 20-Plex Gene Expression Analysis Using Hyperplex PCR (hpPCR) Toward the Development of Salt-Tolerant WheatVariant tracking across Sweden.
Weekly wastewater sampling at up to 22 sites, from October 2022 to December 2023. Adaptable hpPCR panels tracked SARS-CoV-2 mutations alongside qPCR and sequencing.
Water Research · 2025
Swedish University of Agricultural Sciences (SLU), KTH and collaborators, with Aplex Bio.
Read publication: Hyperplex PCR enables highly multiplexed analysis of point mutations in wastewater: Long-term SARS-CoV-2 variant surveillance in Sweden as a case study
APLEX TECHNOLOGY NOTES
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Your question. Your panel.
Choose an existing panel or create a custom one with Panel Foundry.
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